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美國Orbigen
Orbigen位于風(fēng)景如畫(huà)的美國加州SAN DIEGO,其主要成員來(lái)自該地區著(zhù)名的生物科技公司和大學(xué),在真核表達,逆轉錄表達,SiRNA,抗體開(kāi)發(fā)等方面居于世界領(lǐng)先地位。著(zhù)名品牌有:
Sapphire Baculovirus真核表達系統及預制基因庫
Orbigen公司可以提供幾百種重組的桿狀病毒表達載體,其涉及的外源基因主要來(lái)自于人、大鼠、小鼠、酵母、病毒等多種生物。除提供插入單個(gè)外源基因的重組桿狀病毒表達載體外,該公司還可以提供多種插入GST、His6標記融合基因的重組桿狀病毒表達載體,極大地方便了客戶(hù)的科研工作。
Phoenix Retrovirus逆轉錄表達系統
該系統應用非常廣泛,目前世界上有近2500家實(shí)驗室采用該系統轉染將外源基因轉入分化的哺乳動(dòng)物細胞。采用LacZ和GFP基因作為報告系統。
試劑盒組分:
* PhoenixTM ampho 包裝細胞
* PhoenixTM eco 包裝細胞
* 10 μg pBMNZ 質(zhì)粒
* 10 μg pBMN-GFP 質(zhì)粒
BaculoBooster Virus Kit
該試劑盒包括10種不同的桿狀病毒庫,每個(gè)桿狀病毒庫都含有表達一組真核生物伴侶蛋白的重組桿狀病毒混合物,這些伴侶蛋白可以在感染的昆蟲(chóng)細胞(Sf9, Sf21 and T.ni)知道重組蛋白的折疊。該桿狀病毒庫可以與感染細胞內的重組桿狀病毒相互作用,優(yōu)化目的蛋白的表達水平和折疊。
pCMV1/2/3 Xi-Clone 和pIX1/2/3 Xi-Clone 克隆表達系統
pCMV1/2/3 Xi-Clone?系統有3種不同的類(lèi)型,每種類(lèi)型分別包含一種pCMV載體,可以快速高效地實(shí)現體內定向克隆。這類(lèi)pCMV載體含有高度優(yōu)化的細胞巨化病毒(CMV)的啟動(dòng)子/增強子序列,因此表達效率比其它的CMV類(lèi)載體(如:pcDNA3)要高好幾倍。
pIX1/2/3 Xi-Clone?含有有3種不同的類(lèi)型(pIX、pIX2和pIX3),每種類(lèi)型分別包含一種載體,可以快速構建質(zhì)粒模板,實(shí)現體外的快速轉錄和翻譯。該類(lèi)優(yōu)化的載體含有T7啟動(dòng)子,采用Xi-Clone定向克隆技術(shù)。
特點(diǎn):
* 3種不同的配置選擇:不標記表達、N-端HA融合標記和C-端HA融合標記 。采用G418選擇穩定轉染的細胞
* 利用anti-HA抗體可以快速檢測表達蛋白
Orbigen develops, manufactures and markets reagent tools for proteomic research. We are best known for novel antibodies, innovative gene expression technologies, and reliable custom protein expression services. By being at the forefront of technology development, we are proud to provide timely and quality products that help you continuously push the limits of what's possible.
Orbigen is a young and rapidly growing company headquartered in San Diego, California, one of the world's largest clusters of life science research institutes and companies. We employ an extensive group of dynamic and talented scientists who are empowered to create unique and exciting solutions to address your research needs. Many of our scientists are founding members and seasoned veterans from industry leaders. Our experienced technical staff strives to provide high-quality products and expert technical support to life science researchers worldwide.
In addition to providing unique research tools we can also help you commercialize your ideas and reagents. We have extensive experience and proven track record for working with inventors to timely and effectively bring important reagent to market.